{"id":5920,"date":"2023-09-12T02:33:49","date_gmt":"2023-09-12T02:33:49","guid":{"rendered":"https:\/\/cm.vastapps.dev\/tcia-collection\/pca_bx_3dpathology\/"},"modified":"2023-09-13T21:18:06","modified_gmt":"2023-09-13T21:18:06","slug":"pca_bx_3dpathology","status":"publish","type":"tcia_collection","link":"https:\/\/cm.vastapps.dev\/tcia-collection\/pca_bx_3dpathology\/","title":{"rendered":"PCA_BX_3DPATHOLOGY"},"featured_media":7542,"template":"","citation-tax":[],"cancer_types":["Prostate Cancer"],"citations":[2923,2924,2925],"collection_doi":"10.7937\/44MA-GX21","collection_download_info":"Click the Versions tab for more info about data releases.","collection_downloads":[3108,3110],"full_export":"<h1 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-Summary\">Summary<\/h1><span class=\"confluence-embedded-file-wrapper image-right-wrapper confluence-embedded-manual-size\"><img class=\"confluence-embedded-image image-right\" draggable=\"false\" height=\"400\" src=\"https:\/\/wiki.cancerimagingarchive.net\/download\/attachments\/embedded-page\/Public\/3D%20pathology%20of%20prostate%20biopsies%20with%20biochemical%20recurrence%20outcomes:%20raw%20H&amp;E-analog%20datasets%20and%20image%20translation-assisted%20segmentation%20in%203D%20(ITAS3D)%20datasets%20(PCa_Bx_3Dpathology)\/FIG_3.PNG?api=v2\"><\/span><p>This collection provides public access to a 3D pathology dataset of prostate cancer, allowing researchers to further investigate various 3D tissue structures and their correlation with prostate cancer patient outcomes (biochemical recurrence).\u00a0\u00a0 These 3D tissue structures are revealed through: (1) a H&amp;E-analog stain, (2) synthetically generated immunofluorescence staining of CK8 (targeting the luminal epithelial cells of all prostate glands), and (3) 3D segmentation masks of the gland lumen, epithelium, and stromal regions of prostate biopsies.\u00a0 This data collection will promote research in the field of computational 3D pathology for clinical decision support.<\/p><p>In this TCIA collection, we provide the 2x down-sampled fused OTLS-imaged images (H&amp;E-analog staining), the synthetic cytokeratin-8 (CK8) immunofluorescent images at 2x-downsampled resolution, the 3D semantic segmentation masks of glands at 4x down-sampled resolution, the clinical data for patient outcomes (biochemical recurrence), and the coordinates for the cancer-enriched regions of each biopsy. All datasets are from the 50 patient cases studied in this publication: [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34853071\/\" class=\"external-link\" rel=\"nofollow\"><em>W. Xie et al., Cancer Research<\/em>, 2022<\/a>]. \u00a0The Python code for the deep-learning models, and for 3D glandular segmentations based on synthetic-CK8 datasets, are available on GitHub at <a href=\"https:\/\/github.com\/WeisiX\/ITAS3D\" class=\"external-link\" rel=\"nofollow\">https:\/\/github.com\/WeisiX\/ITAS3D<\/a>.<\/p><p style=\"text-align: left;\">Note that the 3D pathology datasets provided in this collection were generated in Dr. Jonathan Liu\u2019s lab at the University of Washington with a custom open-top light-sheet (OTLS) microscope developed by the lab [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31273194\/\" class=\"external-link\" rel=\"nofollow\">A.K. Glaser et al., <em>Nature Communications<\/em>, 2019<\/a>].\u00a0 There is no clinical metadata within the imaging files and all patients are referred to with coded identifiers. \u00a0All of the clinical outcomes data provided in this collection have already been published within the supplement of [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34853071\/\" class=\"external-link\" rel=\"nofollow\"><em>W. Xie et al., Cancer Research<\/em>, 2022<\/a>].<\/p><h3 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-Acknowledgements\"><span>Acknowledgements<\/span><\/h3><p>We would like to acknowledge the individuals and institutions that have provided data for this collection:<\/p><ul><li><p><span style=\"color: rgb(33,37,41);\">Research Program (PCRP) through W81XWH-18-10358 (J.T.C. Liu, L.D. True and J.C. Vaughan), W81XWH-19-1-0589 (N.P. Reder), W81XWH-15-1-0558 (A. Madabhushi) and W81XWH-20-1-0851 (A. Madabhushi and J.T.C. Liu). <\/span><\/p><\/li><li><p><span style=\"color: rgb(33,37,41);\">Support was also provided by the National Cancer Institute (NCI) through K99 CA240681 (A.K. Glaser), R01CA244170 (J.T.C. Liu), U24CA199374 (A. Madabhushi), R01CA249992 (A. Madabhushi), R01CA202752 (A. Madabhushi), R01CA208236 (A. Madabhushi), R01CA216579 (A. Madabhushi), R01CA220581 (A. Madabhushi), R01CA257612 (A. Madabhushi), U01CA239055 (A. Madabhushi), U01CA248226 (A. Madabhushi), and U54CA254566 (A. Madabhushi). <\/span><\/p><\/li><li><p><span style=\"color: rgb(33,37,41);\">Additional support was provided by the National Heart, Lung and Blood Institute (NHLBI) through R01HL151277 (A. Madabhushi), the National Institute of Biomedical Imaging and Bioengineering (NIBIB) through R01EB031002 (J.T.C. Liu) and R43EB028736 (A. Madabhushi), the National Institute of Mental Health through R01MH115767 (J.C. Vaughan), the VA Merit Review Award IBX004121A from the United States Department of Veterans Affairs (A. Madabhushi), the National Science Foundation (NSF) 1934292 HDR: I-DIRSE-FW (J.T.C. Liu), the NSF Graduate Research Fellowships DGE-1762114 (K.W. Bishop) and DGE-1762114 (L. Barner), the Nancy and Buster Alvord Endowment (C.D. Keene), and the Prostate Cancer Foundation Young Investigator Award (N.P. Reder). <\/span><\/p><\/li><li><p><span style=\"color: rgb(33,37,41);\">The training and inference of the deep learning models were facilitated by the advanced computational, storage, and networking infrastructure provided by the Hyak supercomputer system, as funded in part by the student technology fee (STF) at the University of Washington. <\/span><\/p><\/li><li><p><span style=\"color: rgb(33,37,41);\">Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation, the National Institutes of Health, the Department of Defense, the Department of Veterans Affairs, or the United States Government.<\/span><\/p><\/li><\/ul><h3 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-Additionalpublicationsrelatingtothedata\"><span style=\"color: rgb(33,37,41);\"><strong style=\"text-align: left;\" title=\"\">Additional publications relating to the data<\/strong><\/span><\/h3><ul><li><span style=\"color: rgb(33,37,41);\">Renier, Nicolas, et al. &quot;iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging.&quot; Cell 159.4 (2014): 896-910.<\/span><\/li><li><span style=\"color: rgb(33,37,41);\">Glaser, Adam K., et al. &quot;Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues.&quot; Nature communications 10.1 (2019): 1-8.<\/span><\/li><\/ul><p><br\/><\/p><div class=\"tab-style-builtin\"><div class=\"localtabs-macro\"><div class=\"aui-tabs horizontal-tabs\" role=\"application\" data-aui-responsive=\"true\"><ul class=\"tabs-menu\"><li class=\"menu-item bv-localtab  active-tab \"><a href=\"#1457544469ba0f6d7166845d88ea4d96f32f0c097\"><strong>Data Access<\/strong><\/a> <\/li><li class=\"menu-item bv-localtab \"><a href=\"#14575444698a69c5e18ad4bc6830e870bde7ad1e6\"><strong>Detailed Description<\/strong><\/a> <\/li><li class=\"menu-item bv-localtab \"><a href=\"#145754446fe28d6f997f64317a780ac52342f7cd0\"><strong>Citations &amp; Data Usage Policy<\/strong><\/a> <\/li><li class=\"menu-item bv-localtab \"><a href=\"#14575444674ca2fa474884476a4946bc25b29b0ed\"><strong>Versions<\/strong><\/a> <\/li><\/ul><div class=\"tabs-pane  active-pane \" id=\"1457544469ba0f6d7166845d88ea4d96f32f0c097\" active=\"true\" name=\"Data Access\" ><h3 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-DataAccess\">Data Access<\/h3><div class=\"table-wrap\"><table class=\"wrapped relative-table confluenceTable\" style=\"width: 66.6168%;\"><colgroup><col style=\"width: 34.3456%;\"\/><col style=\"width: 38.2429%;\"\/><col style=\"width: 27.3616%;\"\/><\/colgroup><tbody><tr><th class=\"confluenceTh\">Data Type<\/th><th class=\"confluenceTh\">Download all or Query\/Filter<\/th><th class=\"confluenceTh\">License<\/th><\/tr><tr><td class=\"confluenceTd\"><span style=\"color: rgb(33,37,41);\">Tissue Slide Images (HDF5, TIFF, XML 3.8TB)<\/span><\/td><td class=\"confluenceTd\"><div class=\"content-wrapper\"><p>\n\n<span class=\"confluence-embedded-file-wrapper confluence-embedded-manual-size\">\n   <a href=\"https:\/\/faspex.cancerimagingarchive.net\/aspera\/faspex\/external_deliveries\/361?passcode=2b36e2d389e0d0adbf55cd01dd33f05e8d7d0a59\" class=\"external-link\" rel=\"nofollow\"><button class=\"tcia-btn tcia-download-color\"><i class=\"fa fa-cloud-download\" \/> Download<\/button><\/a>\u00a0\n<\/span>\n\n<span class=\"confluence-embedded-file-wrapper confluence-embedded-manual-size\">\n   <a href=\"https:\/\/pathdb.cancerimagingarchive.net\/imagesearch?f[0]=collection:pca_bx_3d\" class=\"external-link\" rel=\"nofollow\"><button class=\"tcia-btn tcia-search-color\"><i class=\"fa fa-search\" \/> Search<\/button><\/a>\u00a0\n<\/span><\/p>(Download and apply the\u00a0<a href=\"https:\/\/www.ibm.com\/aspera\/connect\/\" class=\"external-link\" rel=\"nofollow\">IBM-Aspera-Connect plugin\u00a0<\/a>to your browser to retrieve this faspex package)\u00a0<\/div><\/td><td class=\"confluenceTd\"><div class=\"content-wrapper\"><p>\n<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" class=\"external-link\" rel=\"nofollow\">CC BY 4.0<\/a><\/p><\/div><\/td><\/tr><tr><td class=\"confluenceTd\">Clinical data (CSV)<\/td><td class=\"confluenceTd\"><div class=\"content-wrapper\"><p>\n\n<span class=\"confluence-embedded-file-wrapper confluence-embedded-manual-size\">\n   <a href=\"https:\/\/wiki.cancerimagingarchive.net\/download\/attachments\/145754446\/Biopsy%20list%20with%20BCR%20outcomes%20and%20cancer-enriched%20coordinates.xlsx?api=v2\" rel=\"nofollow\"><button class=\"tcia-btn tcia-download-color\"><i class=\"fa fa-cloud-download\" \/> Download<\/button><\/a>\u00a0\n<\/span><\/p><\/div><\/td><td class=\"confluenceTd\"><div class=\"content-wrapper\"><p>\n<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" class=\"external-link\" rel=\"nofollow\">CC BY 4.0<\/a><\/p><\/div><\/td><\/tr><\/tbody><\/table><\/div><p>Click the Versions tab for more info about data releases.<\/p><h3 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-AdditionalResourcesforthisDataset\">Additional Resources for this Dataset<\/h3><ul><li>The Python code for the deep-learning models, and for 3D glandular segmentations based on synthetic-CK8 datasets, are available on GitHub at <a href=\"https:\/\/github.com\/WeisiX\/ITAS3D\" class=\"external-link\" rel=\"nofollow\">https:\/\/github.com\/WeisiX\/ITAS3D<\/a>.<\/li><\/ul><\/div><div class=\"tabs-pane \" id=\"14575444698a69c5e18ad4bc6830e870bde7ad1e6\" name=\"Detailed Description\" ><h3 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-DetailedDescription\">Detailed Description<\/h3><div class=\"table-wrap\"><table class=\"wrapped confluenceTable\"><colgroup><col\/><col\/><\/colgroup><tbody><tr><th class=\"confluenceTh\"><p>Image Statistics<\/p><\/th><th class=\"confluenceTh\">Pathology Image Statistics<\/th><\/tr><tr><td class=\"confluenceTd\"><p>Modalities<\/p><\/td><td class=\"confluenceTd\"><p>Hierarchical Data Format V5, Tiff stack<\/p><\/td><\/tr><tr><td class=\"confluenceTd\"><p>Number of Patients<\/p><\/td><td class=\"confluenceTd\"><p>50<\/p><\/td><\/tr><tr><td class=\"confluenceTd\"><p>Number of Images<\/p><\/td><td class=\"confluenceTd\"><p>118<\/p><\/td><\/tr><tr><td class=\"confluenceTd\">Images Size (tB)<\/td><td class=\"confluenceTd\">3.8<\/td><\/tr><\/tbody><\/table><\/div><p>Our 3D imaging method is entirely slide-free and non-destructive. \u00a0We image intact tissue specimens (prostate biopsies) that have been labeled with a fluorescent analog of H&amp;E staining and optically cleared to make them transparent to light. The imaging is performed with a custom-developed in-house open-top light-sheet (OTLS) microscope [<a href=\"https:\/\/www.nature.com\/articles\/s41467-019-10534-0\" class=\"external-link\" rel=\"nofollow\">A.K. Glaser et al., <em>Nature Communications<\/em>, 2019<\/a>]. \u00a0<\/p><p>Our OTLS microscope uses an sCMOS camera to collect images of optically cleared tissues in a slice-by-slice manner as we translate the specimens through an illumination \u201clight sheet.\u201d \u00a0The sCMOS cameras generate raw 16-bit TIFF files (grayscale) that are assembled into volumetric imaging \u201ctiles\u201d within the RAM of the acquisition computer. \u00a0These imaging tiles are like volumetric bricks of data, as shown in Fig. 1G of [<a href=\"https:\/\/www.nature.com\/articles\/s41467-019-10534-0\" class=\"external-link\" rel=\"nofollow\">A.K. Glaser et al., <em>Nature Communications<\/em>, 2019<\/a>]. \u00a0Each tile is then saved to the hard drive in the form of a multi-resolution HDF5 file. \u00a0As adjacent imaging tiles are scanned within the specimen, they are appended into the same HDF5 in the hard drive. The HDF5 file is similar to a 3D TIFF stack except it contains multiple downsampled versions of the dataset so that users can quickly visualize or access the 3D data at whatever resolution they need. \u00a0The HDF5 file is also \u201cchunked\u201d so that smaller volumetric regions of interest can be quickly accessed. \u00a0The HDF5 file has an associated XML file that contains microscope metadata (e.g. stage coordinates for each tile, acquisition parameters, etc.).<\/p><p>Before performing computational analysis of the imaging data, we \u201cfuse\u201d the 3D imaging data contained in the raw HDF5 file. \u00a0This is done with an open-source program called \u201cBigStitcher\u201d that finely aligns all of the volumetric imaging tiles, shears the dataset, and blends the edges of those imaging tiles such that a \u201cseamless\u201d 3D image is generated. \u00a0In this project, we also downsample the imaging data by a factor of 2X in all three dimensions during this fusion process. \u00a0The fused dataset is saved as an uncompressed HDF5 file and is used as an input for our downstream ITAS3D segmentation pipeline [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34853071\/\" class=\"external-link\" rel=\"nofollow\"><em>W. Xie et al., Cancer Research<\/em>, 2022<\/a>].\u00a0<\/p><p>The fused HDF5 datasets (2X downsampled compared to the original data) are the rawest form of the imaging data that is provided in this TCIA collection.\u00a0 These are two-channel grayscale volumetric datasets in which one channel is the fluorescent nuclear stain (TO-PRO-3) and the second channel is the eosin stain.\u00a0 The TCIA collection also contains synthetic CK8 immunofluorescence datasets (2X downsampled compared to the original data) that are generated through a deep-learning image-translation model [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34853071\/\" class=\"external-link\" rel=\"nofollow\"><em>W. Xie et al., Cancer Research<\/em>, 2022<\/a>].\u00a0 The CK8 stain labels the luminal epithelial cells of all prostate glands (benign or cancerous).\u00a0 Finally, we provide the segmentation masks of the lumen, epithelial, and stromal compartments of each biopsy (4X downsampled compared to the original data).<\/p><p><br\/><\/p><\/div><div class=\"tabs-pane \" id=\"145754446fe28d6f997f64317a780ac52342f7cd0\" name=\"Citations BITVOODOO_ANDamp; Data Usage Policy\" ><h3 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-Citations&amp;DataUsagePolicy\">Citations &amp; Data Usage Policy<\/h3><p>\n<p>\nUsers must abide by the <a href=\"https:\/\/wiki.cancerimagingarchive.net\/x\/c4hF\" class=\"external-link\" rel=\"nofollow\">TCIA Data Usage Policy and Restrictions<\/a>. Attribution should include references to the following citations:\n<\/p><\/p><div class=\"confluence-information-macro confluence-information-macro-information\"><p class=\"title\">Data Citation<\/p><span class=\"aui-icon aui-icon-small aui-iconfont-info confluence-information-macro-icon\"><\/span><div class=\"confluence-information-macro-body\"><p><span style=\"color: rgb(52,73,94);\">Xie, W., Reder, N. P., Koyuncu, C. F., Leo, P., Hawley, S., Huang, H., Mao, C., POSTUPNA, N. A. D. I. A., kang, soyoung, Serafin, R., Gao, G., Han, Q., Bishop, K., Barner, L., Fu, P., Wright, J., Keene, C., Vaughan, J., Janowczyk, A., \u2026 Liu, J. (2023).<span>\u00a0<\/span><\/span><em>3D pathology of prostate biopsies with biochemical recurrence outcomes: raw H&amp;E-analog datasets and image translation-assisted segmentation in 3D (ITAS3D) datasets (PCa_Bx_3Dpathology)<\/em><span style=\"color: rgb(52,73,94);\"><span>\u00a0<\/span>(Version 1) [Data set]. The Cancer Imaging Archive. <a href=\"https:\/\/doi.org\/10.7937\/44MA-GX21\" class=\"external-link\" rel=\"nofollow\">https:\/\/doi.org\/10.7937\/44MA-GX21<\/a><\/span><\/p><\/div><\/div><div class=\"confluence-information-macro confluence-information-macro-information\"><p class=\"title\">Publication Citation<\/p><span class=\"aui-icon aui-icon-small aui-iconfont-info confluence-information-macro-icon\"><\/span><div class=\"confluence-information-macro-body\"><p><span style=\"color: rgb(33,37,41);\">Xie, W., Reder, N. P., Koyuncu, C., Leo, P., Hawley, S., Huang, H., Mao, C., Postupna, N., Kang, S., Serafin, R., Gao, G., Han, Q., Bishop, K. W., Barner, L. A., Fu, P., Wright, J. L., Keene, C. D., Vaughan, J. C., Janowczyk, A., \u2026 Liu, J. T. C. (2021). Prostate Cancer Risk Stratification via Nondestructive 3D Pathology with Deep Learning\u2013Assisted Gland Analysis. In Cancer Research (Vol. 82, Issue 2, pp. 334\u2013345). American Association for Cancer Research (AACR).<span>\u00a0<\/span><\/span><a style=\"text-decoration: none;text-align: left;\" rel=\"nofollow\" href=\"https:\/\/doi.org\/10.1158\/0008-5472.can-21-2843\" class=\"external-link\">https:\/\/doi.org\/10.1158\/0008-5472.can-21-2843<\/a><\/p><\/div><\/div><div class=\"confluence-information-macro confluence-information-macro-information\"><p class=\"title\">TCIA Citation<\/p><span class=\"aui-icon aui-icon-small aui-iconfont-info confluence-information-macro-icon\"><\/span><div class=\"confluence-information-macro-body\"><p><span style=\"color: rgb(0,0,0);text-decoration: none;\">Clark, K., Vendt, B., Smith, K., Freymann, J., Kirby, J., Koppel, P., Moore, S., Phillips, S., Maffitt, D., Pringle, M., Tarbox, L., &amp; Prior, F. (2013). The Cancer Imaging Archive (TCIA): Maintaining and Operating a Public Information Repository. In Journal of Digital Imaging (Vol. 26, Issue 6, pp. 1045\u20131057). Springer Science and Business Media LLC.<\/span><span style=\"color: rgb(102,102,102);text-decoration: none;\"> <\/span><span style=\"color: rgb(102,102,102);text-decoration: none;\"><a title=\"https:\/\/doi.org\/10.1007\/s10278-013-9622-7\" href=\"https:\/\/doi.org\/10.1007\/s10278-013-9622-7\" class=\"external-link\" rel=\"nofollow\"><span>https:\/\/doi.org\/10.1007\/s10278-013-9622-7<\/span><\/a><\/span><\/p><\/div><\/div><h3 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-OtherPublicationsUsingThisData\">Other Publications Using This Data<\/h3><p><span>TCIA maintains\u00a0<\/span><a href=\"https:\/\/www.cancerimagingarchive.net\/publications\/\" class=\"external-link\" rel=\"nofollow\">a list of publications<\/a><span> which leverage TCIA data. <\/span> If you have a manuscript you'd like to add please<a href=\"http:\/\/www.cancerimagingarchive.net\/support\/\" class=\"external-link\" rel=\"nofollow\"> contact the TCIA Helpdesk<\/a>.<\/p><\/div><div class=\"tabs-pane \" id=\"14575444674ca2fa474884476a4946bc25b29b0ed\" name=\"Versions\" ><h3 id=\"id-3Dpathologyofprostatebiopsieswithbiochemicalrecurrenceoutcomes:rawH&amp;Eanalogdatasetsandimagetranslationassistedsegmentationin3D(ITAS3D)datasets(PCa_Bx_3Dpathology)-Version1(Current):Updated2023\/03\/07\">Version 1 (Current): Updated 2023\/03\/07<\/h3><div class=\"table-wrap\"><table class=\"relative-table wrapped confluenceTable\" style=\"width: 73.5107%;\"><colgroup><col style=\"width: 31.7907%;\"\/><col style=\"width: 37.2413%;\"\/><col style=\"width: 30.9227%;\"\/><\/colgroup><tbody><tr><th class=\"confluenceTh\">Data Type<\/th><th class=\"confluenceTh\">Download all or Query\/Filter<\/th><th class=\"confluenceTh\">License<\/th><\/tr><tr><td class=\"confluenceTd\"><span style=\"color: rgb(33,37,41);\">Tissue Slide Images (HDF5, TIFF, XML 3.8TB)<\/span><\/td><td class=\"confluenceTd\"><div class=\"content-wrapper\"><p>\n\n<span class=\"confluence-embedded-file-wrapper confluence-embedded-manual-size\">\n   <a href=\"https:\/\/faspex.cancerimagingarchive.net\/aspera\/faspex\/external_deliveries\/361?passcode=2b36e2d389e0d0adbf55cd01dd33f05e8d7d0a59\" class=\"external-link\" rel=\"nofollow\"><button class=\"tcia-btn tcia-download-color\"><i class=\"fa fa-cloud-download\" \/> Download<\/button><\/a>\u00a0\n<\/span>\n\n<span class=\"confluence-embedded-file-wrapper confluence-embedded-manual-size\">\n   <a href=\"https:\/\/pathdb.cancerimagingarchive.net\/imagesearch?f[0]=collection:pca_bx_3d\" class=\"external-link\" rel=\"nofollow\"><button class=\"tcia-btn tcia-search-color\"><i class=\"fa fa-search\" \/> Search<\/button><\/a>\u00a0\n<\/span><\/p><span style=\"color: rgb(33,37,41);text-decoration: none;\">(Download requires Aspera plugin<\/span>)<\/div><\/td><td class=\"confluenceTd\"><div class=\"content-wrapper\"><p>\n<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" class=\"external-link\" rel=\"nofollow\">CC BY 4.0<\/a><\/p><\/div><\/td><\/tr><tr><td class=\"confluenceTd\">Clinical data (CSV)<\/td><td class=\"confluenceTd\"><div class=\"content-wrapper\"><p>\n\n<span class=\"confluence-embedded-file-wrapper confluence-embedded-manual-size\">\n   <a href=\"https:\/\/wiki.cancerimagingarchive.net\/download\/attachments\/145754446\/Biopsy%20list%20with%20BCR%20outcomes%20and%20cancer-enriched%20coordinates.xlsx?api=v2\" rel=\"nofollow\"><button class=\"tcia-btn tcia-download-color\"><i class=\"fa fa-cloud-download\" \/> Download<\/button><\/a>\u00a0\n<\/span><\/p><\/div><\/td><td class=\"confluenceTd\"><div class=\"content-wrapper\"><p>\n<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\" class=\"external-link\" rel=\"nofollow\">CC BY 4.0<\/a><\/p><\/div><\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><p><br\/><\/p><p><br\/><\/p>","versions":false,"additional_resources":"<ul><li>The Python code for the deep-learning models, and for 3D glandular segmentations based on synthetic-CK8 datasets, are available on GitHub at <a href=\"https:\/\/github.com\/WeisiX\/ITAS3D\">https:\/\/github.com\/WeisiX\/ITAS3D<\/a>.<\/li><\/ul>","cancer_locations":["Prostate"],"collection_page_accessibility":"Public","publications_related":"<ul><li>Renier, Nicolas, et al. \"iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging.\" Cell 159.4 (2014): 896-910.<\/li><li>Glaser, Adam K., et al. \"Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues.\" Nature communications 10.1 (2019): 1-8.<\/li><\/ul>\n<br\/>","version_change_log":"","version_change_log_archived":"<table><colgroup><col\/><col\/><col\/><\/colgroup><tbody><tr><th>Data Type<\/th><th>Download all or Query\/Filter<\/th><th>License<\/th><\/tr><tr><td>Tissue Slide Images (HDF5, TIFF, XML 3.8TB)<\/td><td><div><p>\n<a href=\"https:\/\/faspex.cancerimagingarchive.net\/aspera\/faspex\/external_deliveries\/361?passcode=2b36e2d389e0d0adbf55cd01dd33f05e8d7d0a59\"><button><i> <\/i> Download<\/button><\/a>\u00a0\n\n\n<a href=\"https:\/\/pathdb.cancerimagingarchive.net\/imagesearch?f[0]=collection:pca_bx_3d\"><button><i> <\/i> Search<\/button><\/a>\u00a0\n<\/p>(Download requires Aspera plugin)<\/div><\/td><td><div><p>\n<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\">CC BY 4.0<\/a><\/p><\/div><\/td><\/tr><tr><td>Clinical data (CSV)<\/td><td><div><p>\n<a download=\"\" href=\"\/wp-content\/uploads\/Biopsy-list-with-BCR-outcomes-and-cancer-enriched-coordinates.xlsx\" target=\"_blank\"><button><i> <\/i> Download<\/button><\/a>\u00a0\n<\/p><\/div><\/td><td><div><p>\n<a href=\"https:\/\/creativecommons.org\/licenses\/by\/4.0\/\">CC BY 4.0<\/a><\/p><\/div><\/td><\/tr><\/tbody><\/table>","analysis_results":"","collection_status":"Complete","publications_using":"TCIA maintains\u00a0<a href=\"https:\/\/www.cancerimagingarchive.net\/publications\/\">a list of publications<\/a> which leverage TCIA data.  If you have a manuscript you'd like to add please<a href=\"http:\/\/www.cancerimagingarchive.net\/support\/\"> contact the TCIA Helpdesk<\/a>.","species":["Human"],"collection_title":"3D pathology of prostate biopsies with biochemical recurrence outcomes: raw H&E-analog datasets and image translation-assisted segmentation in 3D (ITAS3D) datasets","detailed_description":"Our 3D imaging method is entirely slide-free and non-destructive. \u00a0We image intact tissue specimens (prostate biopsies) that have been labeled with a fluorescent analog of H&amp;E staining and optically cleared to make them transparent to light. The imaging is performed with a custom-developed in-house open-top light-sheet (OTLS) microscope [<a href=\"https:\/\/www.nature.com\/articles\/s41467-019-10534-0\">A.K. Glaser et al., <em>Nature Communications<\/em>, 2019<\/a>]. \u00a0\nOur OTLS microscope uses an sCMOS camera to collect images of optically cleared tissues in a slice-by-slice manner as we translate the specimens through an illumination \u201clight sheet.\u201d \u00a0The sCMOS cameras generate raw 16-bit TIFF files (grayscale) that are assembled into volumetric imaging \u201ctiles\u201d within the RAM of the acquisition computer. \u00a0These imaging tiles are like volumetric bricks of data, as shown in Fig. 1G of [<a href=\"https:\/\/www.nature.com\/articles\/s41467-019-10534-0\">A.K. Glaser et al., <em>Nature Communications<\/em>, 2019<\/a>]. \u00a0Each tile is then saved to the hard drive in the form of a multi-resolution HDF5 file. \u00a0As adjacent imaging tiles are scanned within the specimen, they are appended into the same HDF5 in the hard drive. The HDF5 file is similar to a 3D TIFF stack except it contains multiple downsampled versions of the dataset so that users can quickly visualize or access the 3D data at whatever resolution they need. \u00a0The HDF5 file is also \u201cchunked\u201d so that smaller volumetric regions of interest can be quickly accessed. \u00a0The HDF5 file has an associated XML file that contains microscope metadata (e.g. stage coordinates for each tile, acquisition parameters, etc.).\nBefore performing computational analysis of the imaging data, we \u201cfuse\u201d the 3D imaging data contained in the raw HDF5 file. \u00a0This is done with an open-source program called \u201cBigStitcher\u201d that finely aligns all of the volumetric imaging tiles, shears the dataset, and blends the edges of those imaging tiles such that a \u201cseamless\u201d 3D image is generated. \u00a0In this project, we also downsample the imaging data by a factor of 2X in all three dimensions during this fusion process. \u00a0The fused dataset is saved as an uncompressed HDF5 file and is used as an input for our downstream ITAS3D segmentation pipeline [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34853071\/\"><em>W. Xie et al., Cancer Research<\/em>, 2022<\/a>].\u00a0\nThe fused HDF5 datasets (2X downsampled compared to the original data) are the rawest form of the imaging data that is provided in this TCIA collection.\u00a0 These are two-channel grayscale volumetric datasets in which one channel is the fluorescent nuclear stain (TO-PRO-3) and the second channel is the eosin stain.\u00a0 The TCIA collection also contains synthetic CK8 immunofluorescence datasets (2X downsampled compared to the original data) that are generated through a deep-learning image-translation model [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34853071\/\"><em>W. Xie et al., Cancer Research<\/em>, 2022<\/a>].\u00a0 The CK8 stain labels the luminal epithelial cells of all prostate glands (benign or cancerous).\u00a0 Finally, we provide the segmentation masks of the lumen, epithelial, and stromal compartments of each biopsy (4X downsampled compared to the original data).\n<br\/>","related_analysis_results":false,"subjects":"50","collection_short_title":"PCa_Bx_3Dpathology","data_types":["Pathology"],"date_updated":"2023-03-21","collection_browse_title":"","supporting_data":["Clinical"],"collection_featured_image":{"ID":"7542","post_author":"6","post_date":"2023-09-13 03:32:23","post_date_gmt":"2023-09-13 03:32:23","post_content":"","post_title":"FIG_3","post_excerpt":"","post_status":"inherit","comment_status":"open","ping_status":"closed","post_password":"","post_name":"fig_3","to_ping":"","pinged":"","post_modified":"2023-09-13 11:54:00","post_modified_gmt":"2023-09-13 11:54:00","post_content_filtered":"","post_parent":"5920","guid":"https:\/\/cm.vastapps.dev\/wp-content\/uploads\/FIG_3.png","menu_order":"0","post_type":"attachment","post_mime_type":"image\/png","comment_count":"0","pod_item_id":"7542"},"collection_summary":"<p>This collection provides public access to a 3D pathology dataset of prostate cancer, allowing researchers to further investigate various 3D tissue structures and their correlation with prostate cancer patient outcomes (biochemical recurrence).\u00a0\u00a0 These 3D tissue structures are revealed through: (1) a H&amp;E-analog stain, (2) synthetically generated immunofluorescence staining of CK8 (targeting the luminal epithelial cells of all prostate glands), and (3) 3D segmentation masks of the gland lumen, epithelium, and stromal regions of prostate biopsies.\u00a0 This data collection will promote research in the field of computational 3D pathology for clinical decision support.<\/p><p>In this TCIA collection, we provide the 2x down-sampled fused OTLS-imaged images (H&amp;E-analog staining), the synthetic cytokeratin-8 (CK8) immunofluorescent images at 2x-downsampled resolution, the 3D semantic segmentation masks of glands at 4x down-sampled resolution, the clinical data for patient outcomes (biochemical recurrence), and the coordinates for the cancer-enriched regions of each biopsy. All datasets are from the 50 patient cases studied in this publication: [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34853071\/\"><em>W. Xie et al., Cancer Research<\/em>, 2022<\/a>]. \u00a0The Python code for the deep-learning models, and for 3D glandular segmentations based on synthetic-CK8 datasets, are available on GitHub at <a href=\"https:\/\/github.com\/WeisiX\/ITAS3D\">https:\/\/github.com\/WeisiX\/ITAS3D<\/a>.<\/p><p>Note that the 3D pathology datasets provided in this collection were generated in Dr. Jonathan Liu\u2019s lab at the University of Washington with a custom open-top light-sheet (OTLS) microscope developed by the lab [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31273194\/\">A.K. Glaser et al., <em>Nature Communications<\/em>, 2019<\/a>].\u00a0 There is no clinical metadata within the imaging files and all patients are referred to with coded identifiers. \u00a0All of the clinical outcomes data provided in this collection have already been published within the supplement of [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34853071\/\"><em>W. Xie et al., Cancer Research<\/em>, 2022<\/a>].<\/p>","collection_acknowledgements":"We would like to acknowledge the individuals and institutions that have provided data for this collection:\n<ul><li><p>Research Program (PCRP) through W81XWH-18-10358 (J.T.C. Liu, L.D. True and J.C. Vaughan), W81XWH-19-1-0589 (N.P. Reder), W81XWH-15-1-0558 (A. Madabhushi) and W81XWH-20-1-0851 (A. Madabhushi and J.T.C. Liu). <\/p><\/li><li><p>Support was also provided by the National Cancer Institute (NCI) through K99 CA240681 (A.K. Glaser), R01CA244170 (J.T.C. Liu), U24CA199374 (A. Madabhushi), R01CA249992 (A. Madabhushi), R01CA202752 (A. Madabhushi), R01CA208236 (A. Madabhushi), R01CA216579 (A. Madabhushi), R01CA220581 (A. Madabhushi), R01CA257612 (A. Madabhushi), U01CA239055 (A. Madabhushi), U01CA248226 (A. Madabhushi), and U54CA254566 (A. Madabhushi). <\/p><\/li><li><p>Additional support was provided by the National Heart, Lung and Blood Institute (NHLBI) through R01HL151277 (A. Madabhushi), the National Institute of Biomedical Imaging and Bioengineering (NIBIB) through R01EB031002 (J.T.C. Liu) and R43EB028736 (A. Madabhushi), the National Institute of Mental Health through R01MH115767 (J.C. Vaughan), the VA Merit Review Award IBX004121A from the United States Department of Veterans Affairs (A. Madabhushi), the National Science Foundation (NSF) 1934292 HDR: I-DIRSE-FW (J.T.C. Liu), the NSF Graduate Research Fellowships DGE-1762114 (K.W. Bishop) and DGE-1762114 (L. Barner), the Nancy and Buster Alvord Endowment (C.D. Keene), and the Prostate Cancer Foundation Young Investigator Award (N.P. Reder). <\/p><\/li><li><p>The training and inference of the deep learning models were facilitated by the advanced computational, storage, and networking infrastructure provided by the Hyak supercomputer system, as funded in part by the student technology fee (STF) at the University of Washington. <\/p><\/li><li><p>Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation, the National Institutes of Health, the Department of Defense, the Department of Veterans Affairs, or the United States Government.<\/p><\/li><\/ul>","collection_funding":"","hide_from_browse_table":"0","_links":{"self":[{"href":"https:\/\/cm.vastapps.dev\/api\/v1\/collections\/5920"}],"collection":[{"href":"https:\/\/cm.vastapps.dev\/api\/v1\/collections"}],"about":[{"href":"https:\/\/cm.vastapps.dev\/api\/wp\/v2\/types\/tcia_collection"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cm.vastapps.dev\/api\/wp\/v2\/media\/7542"}],"wp:attachment":[{"href":"https:\/\/cm.vastapps.dev\/api\/wp\/v2\/media?parent=5920"}],"wp:term":[{"taxonomy":"tcia_citation_tax","embeddable":true,"href":"https:\/\/cm.vastapps.dev\/api\/v1\/citation-tax?post=5920"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}